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plasmid pgbkt7  (TaKaRa)


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    Structured Review

    TaKaRa plasmid pgbkt7
    Plasmid Pgbkt7, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 4607 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+pgbkt7+bd/pGBKT7+DNA-BD+Vector/pm42827260-412-55-57
    Average 99 stars, based on 4607 article reviews
    plasmid pgbkt7 - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Non-canonical role of a PHOSPHATE1 HOMOLOG 2 in suppressing seedling photomorphogenesis via the TOC1–PIF4 module
    Article Snippet: .. The PHO1;H2 (2424bp) was amplified and digested with Xma1 and BamH1 , followed by ligation into the vector pGBKT7 -BD (containing the GAL4 DNA binding-domain) (Takara Bio, Shiga, Japan) as bait with the intact reading frame. .. The TOC1 (1857 bp) was amplified and digested with EcoRI and XmaI and ligated into pGBKT7 (Takara Bio) containing the GAL4 Activation domain (AD) sequence to serve as prey.

    Ligation:

    Article Title: Non-canonical role of a PHOSPHATE1 HOMOLOG 2 in suppressing seedling photomorphogenesis via the TOC1–PIF4 module
    Article Snippet: .. The PHO1;H2 (2424bp) was amplified and digested with Xma1 and BamH1 , followed by ligation into the vector pGBKT7 -BD (containing the GAL4 DNA binding-domain) (Takara Bio, Shiga, Japan) as bait with the intact reading frame. .. The TOC1 (1857 bp) was amplified and digested with EcoRI and XmaI and ligated into pGBKT7 (Takara Bio) containing the GAL4 Activation domain (AD) sequence to serve as prey.

    Binding Assay:

    Article Title: Non-canonical role of a PHOSPHATE1 HOMOLOG 2 in suppressing seedling photomorphogenesis via the TOC1–PIF4 module
    Article Snippet: .. The PHO1;H2 (2424bp) was amplified and digested with Xma1 and BamH1 , followed by ligation into the vector pGBKT7 -BD (containing the GAL4 DNA binding-domain) (Takara Bio, Shiga, Japan) as bait with the intact reading frame. .. The TOC1 (1857 bp) was amplified and digested with EcoRI and XmaI and ligated into pGBKT7 (Takara Bio) containing the GAL4 Activation domain (AD) sequence to serve as prey.

    Transactivation Assay:

    Article Title: Coordinated control for the auricle asymmetric development by ZmIDD14 and ZmIDD15 fine-tune the high-density planting adaption in maize.
    Article Snippet: The fluorescent signal was visualized using an FV1200 confocal laser scanning microscope (Olympus, Tokyo, Japan) with a 488-nm laser line for GFP and a 552-nm laser line for mCherry. .. For the transactivation assay, the full-length coding sequences of ZmIDD14 and ZmIDD15 were separately cloned into the vector pGBKT7-BD (Biosciences Clontech, Palo Alto, CA, USA) by Sma I and EcoRI (New England BioLabs, Beijing, China) digestion and, then, ligated using the ClonExpress II One Step Cloning Kit (Vazyme Biotech, Nanjing, China). .. The pGBKT7-BD constructs and the empty vector control were transformed into the Saccharomyces cerevisiae strain AH109, respectively (Clontech, Terra, CA, USA).

    Article Title: Coordinated control for the auricle asymmetric development by ZmIDD14 and ZmIDD15 fine‐tune the high‐density planting adaption in maize
    Article Snippet: The fluorescent signal was visualized using an FV1200 confocal laser scanning microscope (Olympus, Tokyo, Japan) with a 488‐nm laser line for GFP and a 552‐nm laser line for mCherry. .. For the transactivation assay, the full‐length coding sequences of ZmIDD14 and ZmIDD15 were separately cloned into the vector pGBKT7‐BD (Biosciences Clontech, Palo Alto, CA, USA) by Sma I and Eco RI (New England BioLabs, Beijing, China) digestion and, then, ligated using the ClonExpress II One Step Cloning Kit (Vazyme Biotech, Nanjing, China). .. The pGBKT7‐BD constructs and the empty vector control were transformed into the Saccharomyces cerevisiae strain AH109, respectively (Clontech, Terra, CA, USA).

    Clone Assay:

    Article Title: Coordinated control for the auricle asymmetric development by ZmIDD14 and ZmIDD15 fine-tune the high-density planting adaption in maize.
    Article Snippet: The fluorescent signal was visualized using an FV1200 confocal laser scanning microscope (Olympus, Tokyo, Japan) with a 488-nm laser line for GFP and a 552-nm laser line for mCherry. .. For the transactivation assay, the full-length coding sequences of ZmIDD14 and ZmIDD15 were separately cloned into the vector pGBKT7-BD (Biosciences Clontech, Palo Alto, CA, USA) by Sma I and EcoRI (New England BioLabs, Beijing, China) digestion and, then, ligated using the ClonExpress II One Step Cloning Kit (Vazyme Biotech, Nanjing, China). .. The pGBKT7-BD constructs and the empty vector control were transformed into the Saccharomyces cerevisiae strain AH109, respectively (Clontech, Terra, CA, USA).

    Article Title: Coordinated control for the auricle asymmetric development by ZmIDD14 and ZmIDD15 fine‐tune the high‐density planting adaption in maize
    Article Snippet: The fluorescent signal was visualized using an FV1200 confocal laser scanning microscope (Olympus, Tokyo, Japan) with a 488‐nm laser line for GFP and a 552‐nm laser line for mCherry. .. For the transactivation assay, the full‐length coding sequences of ZmIDD14 and ZmIDD15 were separately cloned into the vector pGBKT7‐BD (Biosciences Clontech, Palo Alto, CA, USA) by Sma I and Eco RI (New England BioLabs, Beijing, China) digestion and, then, ligated using the ClonExpress II One Step Cloning Kit (Vazyme Biotech, Nanjing, China). .. The pGBKT7‐BD constructs and the empty vector control were transformed into the Saccharomyces cerevisiae strain AH109, respectively (Clontech, Terra, CA, USA).

    Cloning:

    Article Title: Coordinated control for the auricle asymmetric development by ZmIDD14 and ZmIDD15 fine-tune the high-density planting adaption in maize.
    Article Snippet: The fluorescent signal was visualized using an FV1200 confocal laser scanning microscope (Olympus, Tokyo, Japan) with a 488-nm laser line for GFP and a 552-nm laser line for mCherry. .. For the transactivation assay, the full-length coding sequences of ZmIDD14 and ZmIDD15 were separately cloned into the vector pGBKT7-BD (Biosciences Clontech, Palo Alto, CA, USA) by Sma I and EcoRI (New England BioLabs, Beijing, China) digestion and, then, ligated using the ClonExpress II One Step Cloning Kit (Vazyme Biotech, Nanjing, China). .. The pGBKT7-BD constructs and the empty vector control were transformed into the Saccharomyces cerevisiae strain AH109, respectively (Clontech, Terra, CA, USA).

    Article Title: Coordinated control for the auricle asymmetric development by ZmIDD14 and ZmIDD15 fine‐tune the high‐density planting adaption in maize
    Article Snippet: The fluorescent signal was visualized using an FV1200 confocal laser scanning microscope (Olympus, Tokyo, Japan) with a 488‐nm laser line for GFP and a 552‐nm laser line for mCherry. .. For the transactivation assay, the full‐length coding sequences of ZmIDD14 and ZmIDD15 were separately cloned into the vector pGBKT7‐BD (Biosciences Clontech, Palo Alto, CA, USA) by Sma I and Eco RI (New England BioLabs, Beijing, China) digestion and, then, ligated using the ClonExpress II One Step Cloning Kit (Vazyme Biotech, Nanjing, China). .. The pGBKT7‐BD constructs and the empty vector control were transformed into the Saccharomyces cerevisiae strain AH109, respectively (Clontech, Terra, CA, USA).

    Article Title: Importin-β From the Recretohalophyte Limonium bicolor Enhances Salt Tolerance in Arabidopsis thaliana by Reducing Root Hair Development and Abscisic Acid Sensitivity
    Article Snippet: .. The ORF of LbSAD2 was introduced into the vector pGBKT7/BD using Ndel digestion sites according to the instruction of an In-Fusion HD Cloning Kit (Clontech Laboratories, Inc.). .. The vectors pGBKT7/BD (empty control), pGBKT7- LbSAD2 (experimental group), pGBKT7-lam (negative control), and pGBKT7- LbTTG1 (positive control) were separately transformed into Y2H Gold yeast ( Saccharomyces cerevisiae ) cells using the Yeastmaker Yeast Transformation System 2 (TaKaRa).



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    TaKaRa gal4 dna binding domain
    Yeast 2‐hybrid interaction assay of the interaction between (a) TrAP from ToLCNDV‐C ([C]), ToLCNDV‐T&C ([T&C]), ToLCKV‐T ([T]) and ToLCNDV‐C[M9] ([C‐M9]) and SlAGO1, CsAGO1, Slrgs‐CaM and SlATG7. (b) REn from ToLCNDV‐C, ToLCNDV‐T&C, ToLCKV‐T and ToLCNDV‐C[M9] and SlPolA, SlRBR, CsPCNA and SlPCNA. TrAP was expressed as a <t>GAL4‐activating</t> domain fusion protein in HGold yeast and mated with Y187 yeast expressing the plant protein as a fusion protein to the GAL4‐DNA binding domain. The figure shows the mated yeast plated on triple dropout (−Trp/−Leu/−His) plates as selection for interaction. Empty vector controls are HGold expressing the empty vector pGADT7‐AD or Y187 expressing the empty pGBKT7‐BD vector.
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    Image Search Results


    Yeast 2‐hybrid interaction assay of the interaction between (a) TrAP from ToLCNDV‐C ([C]), ToLCNDV‐T&C ([T&C]), ToLCKV‐T ([T]) and ToLCNDV‐C[M9] ([C‐M9]) and SlAGO1, CsAGO1, Slrgs‐CaM and SlATG7. (b) REn from ToLCNDV‐C, ToLCNDV‐T&C, ToLCKV‐T and ToLCNDV‐C[M9] and SlPolA, SlRBR, CsPCNA and SlPCNA. TrAP was expressed as a GAL4‐activating domain fusion protein in HGold yeast and mated with Y187 yeast expressing the plant protein as a fusion protein to the GAL4‐DNA binding domain. The figure shows the mated yeast plated on triple dropout (−Trp/−Leu/−His) plates as selection for interaction. Empty vector controls are HGold expressing the empty vector pGADT7‐AD or Y187 expressing the empty pGBKT7‐BD vector.

    Journal: Molecular Plant Pathology

    Article Title: Viral and Host Factors Involved in Host Gain and Host Loss by Tomato Leaf Curl Begomoviruses in Tomato and Cucumbers

    doi: 10.1111/mpp.70202

    Figure Lengend Snippet: Yeast 2‐hybrid interaction assay of the interaction between (a) TrAP from ToLCNDV‐C ([C]), ToLCNDV‐T&C ([T&C]), ToLCKV‐T ([T]) and ToLCNDV‐C[M9] ([C‐M9]) and SlAGO1, CsAGO1, Slrgs‐CaM and SlATG7. (b) REn from ToLCNDV‐C, ToLCNDV‐T&C, ToLCKV‐T and ToLCNDV‐C[M9] and SlPolA, SlRBR, CsPCNA and SlPCNA. TrAP was expressed as a GAL4‐activating domain fusion protein in HGold yeast and mated with Y187 yeast expressing the plant protein as a fusion protein to the GAL4‐DNA binding domain. The figure shows the mated yeast plated on triple dropout (−Trp/−Leu/−His) plates as selection for interaction. Empty vector controls are HGold expressing the empty vector pGADT7‐AD or Y187 expressing the empty pGBKT7‐BD vector.

    Article Snippet: Full‐length ORFs of REn were obtained from ToLCNDV‐C, ToLCNDV‐C[M9], ToLCNDV‐T&C and ToLCKV‐T plasmids and cloned into pGBKT7‐BD, fusing with the Gal4 DNA‐binding domain (Takara: 630443).

    Techniques: Expressing, Binding Assay, Selection, Plasmid Preparation